Flow Cytometry
BD FACSVerseTM
Flow cytometry (FC) is an essential tool for performing a multiparametric single cell analysis. In our lab, FC is utilized for the cytotoxicity analysis of nanomaterials and for studying the interaction of cells with nanomaterials
Analyses and assays
- Cell viability/cytotoxicity using Calcein/ Propidium Iodide or Ethidium Homodimer
- Detection of apoptosis using BD Anexin V FITC assay
- Cell cycle analysis using DNA-BD kit
- Surface markers analysis
Lasers and set up
3-laser, 8-color (4-2-2) configuration
- 488 nm for cell surface markers, live/dead discrimination, cell cycle (SSC, FITC, AlexaFluor488, PE, PI, PerCP, PerCP-Cy5.5, PE-Cy5, 7-AAD, PE-Cy7)
- 640 nm for cell surface markers (APC, AlexaFluor647, APC-Cy7, APC-H7)
- 405 nm for cell surface markers, live-dead discrimination, cell cycle ( BD Horizon V450, Pacific Blue, DPAI, Violet proliferation Dye, Brilliant Violet 421)